ISO 19827:2016(E) specifies a method for the determination of the lethal effects of toxicants to Brachionus calyciflorus after 24 h exposure.

The method is applicable to:

a) chemical substances which are soluble, or which can be maintained as a stable suspension of dispersions under the conditions of the test;

b) industrial or sewage effluents, treated or untreated, if appropriate after decantation, filtration or centrifugation;

c) freshwaters;

d) aqueous extracts.