This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter of cigarette mainstream smoke with an intense smoking regime using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent.

This method is specified using ISO 20778 smoking parameters. An alternative method for the determination of B[a]P is specified in ISO 23906‑1, with a different clean-up using methanol solvent.