Méthodes horizontales pour l'analyse moléculaire de biomarqueurs — Méthodes d'analyse pour la détection des organismes génétiquement modifiés et des produits dérivés — Partie 9: Méthode de criblage construit spécifique basée sur la PCR en temps réel pour la détection de la séquence ADN P35S-nptII

This document specifies a procedure for the detection of the DNA transition sequence between the 35S promoter region from cauliflower mosaic virus (P35S) and the neomycin-phosphotransferase gene (nptII) from the Tn5 transposon of Escherichia coli. The P35S-nptII segment is part of a construct which confers resistance to neomycin/kanamycin antibiotics frequently found in genetically modified (GM) plants.

The detection method is based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific GM plant (event) a follow-up analysis has to be carried out.

This method is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.